Integrating bipolar electrochemistry and electrochemiluminescence imaging with microdroplets for chemical analysis.
Identifieur interne : 000130 ( Main/Exploration ); précédent : 000129; suivant : 000131Integrating bipolar electrochemistry and electrochemiluminescence imaging with microdroplets for chemical analysis.
Auteurs : RBID : pubmed:24140829Abstract
Here we develop a microdroplet sensor based on bipolar electrochemistry and electrochemiluminescence (ECL) imaging. The sensor was constructed with a closed bipolar cell on a hybrid poly(dimethylsioxane) (PDMS)-indium tin oxide (ITO) glass microchip. The ITO microband functions as the bipolar electrode and its two poles are placed in two spatially separate micro-reservoirs predrilled on the PDMS cover. After loading microliter-sized liquid droplets of tris(2,2'-bipyridyl) ruthenium (II)/2-(dibutylamino) ethanol (Ru(bpy)3(2+)/DBAE) and the analyte to the micro-reservoirs, an appropriate external voltage imposed on the driving electrodes could induce the oxidation of Ru(bpy)3(2+)/DBAE and simultaneous reduction of the analyte at the anodic and cathodic poles, respectively. ECL images generated by Ru(bpy)3(2+)/DBAE oxidation at the anodic pole and the electrical current flowing through the bipolar electrode can be recorded for quantitative analyte detection. Several types of quinones were selected as model analytes to demonstrate the sensor performance. Furthermore, the cathodic pole of bipolar electrode can be modified with (3-aminopropyl)triethoxysilane-gold nanoparticles-horseradish peroxidase composites for hydrogen peroxide detection. This microdroplet sensor with a closed bipolar cell can avoid the interference and cross-contamination between analyte solutions and ECL reporting reagents. It is also well adapted for chemical analysis in the incompatible system, e.g., detection of organic compounds insoluble in water by aqueous ECL generation. Moreover, this microdroplet sensor has advantages of simple structure, high sensitivity, fast response and wide dynamic response, providing great promise for chemical and biological analysis.
DOI: 10.1016/j.bios.2013.09.042
PubMed: 24140829
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Integrating bipolar electrochemistry and electrochemiluminescence imaging with microdroplets for chemical analysis.</title><author><name sortKey="Wu, Suozhu" uniqKey="Wu S">Suozhu Wu</name><affiliation wicri:level="1"><nlm:affiliation>Center for High-Performance and Novel Materials, Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center for High-Performance and Novel Materials, Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058</wicri:regionArea></affiliation></author><author><name sortKey="Zhou, Zhenyu" uniqKey="Zhou Z">Zhenyu Zhou</name></author><author><name sortKey="Xu, Linru" uniqKey="Xu L">Linru Xu</name></author><author><name sortKey="Su, Bin" uniqKey="Su B">Bin Su</name></author><author><name sortKey="Fang, Qun" uniqKey="Fang Q">Qun Fang</name></author></titleStmt><publicationStmt><date when="2014">2014</date><idno type="RBID">pubmed:24140829</idno><idno type="pmid">24140829</idno><idno type="doi">10.1016/j.bios.2013.09.042</idno><idno type="wicri:Area/Main/Corpus">000353</idno><idno type="wicri:Area/Main/Curation">000353</idno><idno type="wicri:Area/Main/Exploration">000130</idno></publicationStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Here we develop a microdroplet sensor based on bipolar electrochemistry and electrochemiluminescence (ECL) imaging. The sensor was constructed with a closed bipolar cell on a hybrid poly(dimethylsioxane) (PDMS)-indium tin oxide (ITO) glass microchip. The ITO microband functions as the bipolar electrode and its two poles are placed in two spatially separate micro-reservoirs predrilled on the PDMS cover. After loading microliter-sized liquid droplets of tris(2,2'-bipyridyl) ruthenium (II)/2-(dibutylamino) ethanol (Ru(bpy)3(2+)/DBAE) and the analyte to the micro-reservoirs, an appropriate external voltage imposed on the driving electrodes could induce the oxidation of Ru(bpy)3(2+)/DBAE and simultaneous reduction of the analyte at the anodic and cathodic poles, respectively. ECL images generated by Ru(bpy)3(2+)/DBAE oxidation at the anodic pole and the electrical current flowing through the bipolar electrode can be recorded for quantitative analyte detection. Several types of quinones were selected as model analytes to demonstrate the sensor performance. Furthermore, the cathodic pole of bipolar electrode can be modified with (3-aminopropyl)triethoxysilane-gold nanoparticles-horseradish peroxidase composites for hydrogen peroxide detection. This microdroplet sensor with a closed bipolar cell can avoid the interference and cross-contamination between analyte solutions and ECL reporting reagents. It is also well adapted for chemical analysis in the incompatible system, e.g., detection of organic compounds insoluble in water by aqueous ECL generation. Moreover, this microdroplet sensor has advantages of simple structure, high sensitivity, fast response and wide dynamic response, providing great promise for chemical and biological analysis.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="In-Process"><PMID Version="1">24140829</PMID><DateCreated><Year>2013</Year><Month>11</Month><Day>26</Day></DateCreated><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-4235</ISSN><JournalIssue CitedMedium="Internet"><Volume>53</Volume><PubDate><Year>2014</Year><Month>Mar</Month><Day>15</Day></PubDate></JournalIssue><Title>Biosensors & bioelectronics</Title><ISOAbbreviation>Biosens Bioelectron</ISOAbbreviation></Journal><ArticleTitle>Integrating bipolar electrochemistry and electrochemiluminescence imaging with microdroplets for chemical analysis.</ArticleTitle><Pagination><MedlinePgn>148-53</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.bios.2013.09.042</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0956-5663(13)00660-X</ELocationID><Abstract><AbstractText>Here we develop a microdroplet sensor based on bipolar electrochemistry and electrochemiluminescence (ECL) imaging. The sensor was constructed with a closed bipolar cell on a hybrid poly(dimethylsioxane) (PDMS)-indium tin oxide (ITO) glass microchip. The ITO microband functions as the bipolar electrode and its two poles are placed in two spatially separate micro-reservoirs predrilled on the PDMS cover. After loading microliter-sized liquid droplets of tris(2,2'-bipyridyl) ruthenium (II)/2-(dibutylamino) ethanol (Ru(bpy)3(2+)/DBAE) and the analyte to the micro-reservoirs, an appropriate external voltage imposed on the driving electrodes could induce the oxidation of Ru(bpy)3(2+)/DBAE and simultaneous reduction of the analyte at the anodic and cathodic poles, respectively. ECL images generated by Ru(bpy)3(2+)/DBAE oxidation at the anodic pole and the electrical current flowing through the bipolar electrode can be recorded for quantitative analyte detection. Several types of quinones were selected as model analytes to demonstrate the sensor performance. Furthermore, the cathodic pole of bipolar electrode can be modified with (3-aminopropyl)triethoxysilane-gold nanoparticles-horseradish peroxidase composites for hydrogen peroxide detection. This microdroplet sensor with a closed bipolar cell can avoid the interference and cross-contamination between analyte solutions and ECL reporting reagents. It is also well adapted for chemical analysis in the incompatible system, e.g., detection of organic compounds insoluble in water by aqueous ECL generation. Moreover, this microdroplet sensor has advantages of simple structure, high sensitivity, fast response and wide dynamic response, providing great promise for chemical and biological analysis.</AbstractText><CopyrightInformation>© 2013 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Suozhu</ForeName><Initials>S</Initials><Affiliation>Center for High-Performance and Novel Materials, Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China.</Affiliation></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Zhenyu</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Linru</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Su</LastName><ForeName>Bin</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Fang</LastName><ForeName>Qun</ForeName><Initials>Q</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>09</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biosens Bioelectron</MedlineTA><NlmUniqueID>9001289</NlmUniqueID><ISSNLinking>0956-5663</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Bipolar electrochemistry</Keyword><Keyword MajorTopicYN="N">Electrochemiluminescence imaging</Keyword><Keyword MajorTopicYN="N">Hydrogen peroxide</Keyword><Keyword MajorTopicYN="N">Microdroplet</Keyword><Keyword MajorTopicYN="N">Quinones</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>6</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>8</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>9</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2013</Year><Month>9</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>10</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>10</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>10</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24140829</ArticleId><ArticleId IdType="pii">S0956-5663(13)00660-X</ArticleId><ArticleId IdType="doi">10.1016/j.bios.2013.09.042</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=IndiumV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000130 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000130 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= *** parameter Area/wikiCode missing ***
|area= IndiumV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:24140829
|texte= Integrating bipolar electrochemistry and electrochemiluminescence imaging with microdroplets for chemical analysis.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:24140829" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a IndiumV2
| This area was generated with Dilib version V0.5.76. |  |